CD54 ; Cell surface glycoprotein P3.58 ; Human rhinovirus receptor ; ICAM-1 ; Intercellular adhesion molecule 1 ; Ly 47 ; Major group rhinovirus receptor ; MALA2 ; MyD10 ; Surface antigen of activated B cells
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
19p13.3-p13.2
Clonality
Monoclonal
Description
Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
Host
Mouse
Immunogen
Raji Burkitt lymphoma cells
Isotype
IgG2b Kappa
Positive Control
Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P05362
Uniprot
643447
Gene Id
3383
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
Functional Studies (Order Ab without BSA & Azide); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
2-4μg/ml for 30 minutes at RT
Reviews of Anti-CD54 / ICAM-1 Antibody BHA10202011