Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-419 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp�s), more appropriately called as stress response proteins (srp�s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
Host
Mouse
Immunogen
Recombinant human HSP60 protein
Isotype
IgG1 Kappa
Positive Control
HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA at 1.0mg/ml
Applications
FACS, IF, WB
Description
Optimal dilution for a specific application should be determined., FACS ; IF ; WB
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
WB
0.25-0.5μg/ml
Reviews of Anti-HSP60 (Heat Shock Protein 60) Antibody BHA10201964