Forkhead box protein A1 (FOXA1) ; Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A) ; Transcription factor 3A (TCF-3A or TCF3A)
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
14q21.1
Clonality
Monoclonal
Description
The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
Host
Mouse
Immunogen
Recombinant human FOXA1 protein fragment (aa467-614) (exact sequence is proprietary)
Isotype
IgG2b Kappa
Positive Control
HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.
Reactivity
Human, Rat
Recombinant
FALSE
Regulatory
RUO
Swissprot
P55317
Uniprot
163484
Gene Id
3169
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
WB
0.5-1μg/ml
IHC-F
0.5-1.0μg/ml for 30 minutes at RT
Reviews of Anti-FOXA1 / HNF3A Antibody BHA10201904