Anti-Histone H1 Antibody BHA10201732

Mfr.No.	3005-MSM1X-P1ABX
Alternative Names	H1(0) ; H1.1 ; H1.2 ; H1.3 ; H1.4 ; H1.5 ; H10 ; H1A ; H1F0 ; H1F1
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	3q21-q22; 6q21-q22; 22q13.1
Clonality	Monoclonal
Description	Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Host	Mouse
Immunogen	Nuclei of human leukemia biopsy cells
Isotype	IgG2a Kappa
Positive Control	HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
Reactivity	Human, Mouse, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P07305
Uniprot	226117; 97358
Gene Id	3005

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10201732	3005-MSM1X-P1ABX	100µg	$393.20		Add to Cart

Anti-Histone H1 Antibody BHA10201732

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