Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
Host
Mouse
Immunogen
Recombinant human FTL protein fragment (aa 38-165) (exact sequence is proprietary)
Isotype
IgG1
Positive Control
HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P02792
Uniprot
433670
Gene Id
2512
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.1-0.2μg/million cells
IF
0.1-0.2μg/ml
IHC-F
0.1-0.2μg/ml for 30 min at RT
No Description Available.
No Description Available.
Reviews of Anti-Ferritin, Light Chain (FTL) Antibody BHA10201361