Astrocyte or Intermediate Filament Protein ; Glial Fibrillary Acidic Protein (GFAP)
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
17q21.31
Clonality
Monoclonal
Description
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Host
Mouse
Immunogen
GFAP isolated from pig spinal cord
Isotype
IgG1
Positive Control
Brain or Astrocytoma
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken
Recombinant
FALSE
Regulatory
RUO
Swissprot
P14136
Uniprot
514227
Gene Id
2670
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F