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Home / Primary Antibodies / Flow Cytometry (FACS)

Anti-CD32 (Fc Gamma RIIa) Antibody BHA10201071

SKU : BHA10201071

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NeoBiotechnologies, Inc.

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Mfr.No.	2212-MSM2-P1ABX
Alternative Names	CD32A ; Fc fragment of IgG low affinity IIa receptor ; Fc gamma RIIa ; Fc-gamma RII-a ; Fc-gamma-RIIa ; FCGR2A ; FcgRII ; Fcr-2 ; FcRII-a ; IGFR2
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	1q23
Clonality	Monoclonal
Description	This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (Fc?RII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
Host	Mouse
Immunogen	K562 and FcgRII+L cells
Isotype	IgG1 Kappa
Positive Control	K-562 cells. Lymph node, tonsil and placenta.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P12318
Uniprot	352642
Gene Id	2212

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF

Description

Functional Studies (Order Ab without BSA & Azide); Optimal dilution for a specific application should be determined., FACS ; IF

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA10201071	2212-MSM2-P1ABX	100µg	$393.20		Add to Cart

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