FcRIIIa ; FCR-10 ; FcRIII ; FCGRIII ; FcR-10 ; Fc fragment of IgG, low affinity IIIa, receptor for (CD16) ; neutrophil-specific antigen NA ; CD16a ; low affinity immunoglobulin gamma Fc region receptor III-A ; Fc-gamma RIIIa
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
1q23
Clonality
Monoclonal
Description
It recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. CD16 is represented by two similar genes, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Host
Mouse
Immunogen
Human CD16
Isotype
IgM
Positive Control
K-562 or U-937 cells. Lymph nodes and tonsils.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P08637
Uniprot
372679
Gene Id
2214
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF
Description
Optimal dilution for a specific application should be determined., FACS ; IF
Dilution
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
Reviews of Anti-CD16 / Fcγ Receptor III Antibody BHA10201081