Anti-MART-1 / Melan-A / MLANA Antibody BHA10201194

Mfr.No.	2315-RBP10-P1ABX
Alternative Names	Antigen LB39-AA ; Antigen SK29-AA ; Melanoma antigen recognized by T-cells 1 ; MLAN-A ; MLANA
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	9p24.1
Clonality	Polyclonal
Description	This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Host	Rabbit
Immunogen	Recombinant human full-length MLANA protein
Isotype	IgG Kappa
Positive Control	SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Reactivity	Human, Mouse, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q16655
Uniprot	154069
Gene Id	2315

Buffer	200μg/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells
IF	0.5-1μg/ml
WB	0.5-1.0μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10201194	2315-RBP10-P1ABX	100µg	$393.20		Add to Cart

Anti-MART-1 / Melan-A / MLANA Antibody BHA10201194

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