Anti-AFP (Alpha Fetoprotein) Antibody BHA10200730

Mfr.No.	174-MSM4-P1ABX
Alternative Names	Alpha fetoglobulin ; FETA ; HPAFP
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	4q13.3
Clonality	Monoclonal
Description	It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.Ā
Host	Mouse
Immunogen	Recombinant full-length human Alpha fetoprotein
Isotype	IgG1 Kappa
Positive Control	Hep-G2 cells. Fetal liver or hepatocellular carcinoma
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P02771
Uniprot	518808
Gene Id	174

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-F	1-2μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10200730	174-MSM4-P1ABX	100µg	$393.20		Add to Cart

Anti-AFP (Alpha Fetoprotein) Antibody BHA10200730

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