SERPINA3 ; AACT ; ACT ; Alpha-1-antichymotrypsin ; Antichymotrypsin ; Cell growth-inhibiting gene 24/25 protein ; GIG24 ; GIG25 ; Growth inhibiting protein 24 ; Growth inhibiting protein 25
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
14q32.1
Clonality
Monoclonal
Description
It recognizes a protein of 65-76kDa, which is identified antichymotrypsin (AACT). AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of α 1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants; their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.
Host
Mouse
Immunogen
Recombinant human Antichymotrypsin (AACT) protein fragment (aa49-187) (exact sequence is proprietary)
Isotype
IgG1
Positive Control
HeLa Cells. Tonsil, Pancreas or Histiocytoma.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01011
Uniprot
534293; 710488
Gene Id
12
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
WB
0.5-1.0μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-Alpha-1-Antichymotrypsin (SERPINA3) Antibody BHA10200492