It recognizes a muco-protein of 38-43kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.
Host
Mouse
Immunogen
Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)
Isotype
IgG1 Kappa
Positive Control
HeLa Cells. Angiosarcoma or Mesothelioma.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
Q86YL7
Uniprot
468675
Gene Id
10630
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
WB
0.5-1.0μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-Podoplanin (PDPN) Antibody BHA10200266