Activating Fragment 1 ; CAP20 ; CDK-interacting protein 1 ; CDKI ; CDKN1 ; CDKN1A ; CIP1 ; Cyclin-dependent kinase inhibitor 1A (p21,Cip1) ; DNA Synthesis Inhibitor ; MDA6
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
6p21.31
Clonality
Monoclonal
Description
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. It is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Host
Mouse
Immunogen
Human recombinant p21 protein
Isotype
IgG1 Kappa
Positive Control
HeLa Cells. Skin, colon, or breast carcinoma.
Reactivity
Human, Monkey, Chimpanzee, Mouse, Rat
Recombinant
FALSE
Regulatory
RUO
Swissprot
P38936
Uniprot
370771
Gene Id
1026
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
1-2μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-p21WAF1 (Tumor Suppressor Protein) Antibody BHA10200075