Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody BHA10200105

Mfr.No.	1027-MSM3-P0
Alternative Names	CDKN1B ; CDKN4 ; Cyclin Dependent Kinase Inhibitor 1B ; Cyclin-dependent kinase inhibitor p27 Kip1 ; KIP1 ; MEN1B ; MEN4
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	12p13.1
Clonality	Monoclonal
Description	Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Host	Mouse
Immunogen	Mouse recombinant p27 protein
Isotype	IgG1 Kappa
Positive Control	ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
Reactivity	Human, Monkey, Mouse, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P46527
Uniprot	238990
Gene Id	1027

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution