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Home / Fish Probe

U6 Probe

SKU : BHR15100242

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BioGenex Laboratories

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Mfr.No.	PR031-100
Intended Use	The U6 fluorescenated oligo probe is designed to bind to human U6small nuclear RNA in formalin-fixed, paraffin-embedded humantissues or freshly prepared frozen tissues by in situ hybridization. Thisprobe does not react with normal human mRNA or nuclear DNApresent in tissues. This probe can be used as positive control for in situhybridization assays.
Description	U6 snRNA is the non-coding small nuclear RNA (snRNA) component of U6 snRNP (small nuclear ribonucleoprotein). The U6 snRNA sequence is highly conserved and the function of the U6 snRNA has remained crucial and unchanged through evolution. The U6 cellular transcript is available in abundance with intranuclear distribution in cell/tissue.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acid sequences directly within a cell or tissue. High specificity is ensured through the action of annealing of probe nucleic acid sequence to complementary target nucleic acid sequence. ISH techniques can be used to identify infectious agents in tissue sections, to localize gene expression within individual cells, or to detect specific nucleic acid sequences in cells. In ISH, fixed tissue sections are treated with nucleic acid retrieval solution to expose target nucleic acid sequences. A hapten (fluorescein labeled probe) is hybridized to the exposed target nucleic acid sequences in the cells. Subsequent washing steps remove any probe that is not bound or that is non-specifically bound to the tissue section. An immunohistochemical (IHC) procedure is then used to detect the probe-target hybrid. (Downstream detection of hybridized hapten labeled probe is done by using specific anti-hapten antibody). This procedure includes incubating the slide with a mouse antifluorescein or digoxigenin antibody, followed by detection of this antibody with a second antibody enzyme conjugate. After addition of an appropriate substrate for the enzyme (such as DAB, diaminobenzidine solution), a brown colored reaction product is precipitated at the location of the probe-target hybrid. Microscopic examination of the slide provides visual interpretation of the staining results.
Storage	Store the probe at 2-8o C. The probe is allowed to reach roomtemperature prior to use.This probe is suitable for use till expiry date when stored at 2-8°C. If reagents are stored under any conditions other than those specified inthe package insert, they must be verified by the user.Positive and negative controls should be run simultaneously for everyexperiment. If unexpected staining is observed which cannot beexplained by variations in laboratory procedures and a problem withthe antibody is suspected, contact BioGenex Technical Support at 1-800-421-4149 or your local distributor.

Reference	Datasheet SDS

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100242	PR031-100	25 Tests - Manual	$293		Add to Cart

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