JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Fish Probe

eFISH RB1/13Q12 Dual Color Probe

SKU : BHR15100192

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BioGenex Laboratories

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	FP079-10X
Gene Name	RB1/13Q12
Fluorophore	Orange/Green
Chromosome	13
Intended Use	The BioGenex eFISH RB1/13q12 Dual Color Probe currently available for Research useonly.eFISHRB1/13q12 Dual Color Probe is designed to be used for the detection of thehuman RB1 gene as well as chromosome 13 specific sequences in formalin-fixed,paraffin-embedded tissue or cells by fluorescence in situhybridization (FISH).eFISH BioGenex RB1/13q12 Dual Color Probe in hybridization buffer. The probecontains orange-labeled polynucleotides (Orange: excitation at 547 nm and emission at572 nm, similar to rhodamine), which target the RB1 gene in 13q14.2, and green-labeledpolynucleotides (Green: excitation at 503 nm and emission at 528 nm, similar to FITC),which target sequences of chromosome 13 in the chromosomal region 13q12.11.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or within individual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/anti-fade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acid sequences directly within a cell or tissue. High specificity is ensured through the action of annealing of fluorescence probe nucleic acid sequence to complementary target nucleic acid sequence. ISH techniques can be used to identify genetic aberrations like deletions, amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH BioGenex RB1/13q12 Dual Color Probemust be stored at 2-8°C protected from light and is stable through the expiry date printed on the label.

Reference	Datasheet SDS

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHR15100192	FP079-10X	10 tests	$1152		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.