JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Fish Probe

eFISH PIK3CA/CEN 3 Dual Color Probe

SKU : BHR15100152

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BioGenex Laboratories

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	FP059-10X
Gene Name	PIK3CA/CEN 3
Fluorophore	Orange/Green
Chromosome	3
Intended Use	The BioGenex eFISH PIK3CA/CEN 3Dual Color Probe is currently available forResearch use only.eFISH PIK3CA/CEN 3 Dual Color Probe is designed to detect humanPIK3CA gene as well aschromosome 3 alpha-satellitesin formalin-fixed, paraffinembedded tissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH PIK3CA/CEN 3 Dual Color Probecomes in Ready to use formatinhybridization buffer. The probe contains green-labeled polynucleotides (Green: excitationat 503 nm and emission at 528 nm, similar to FITC which target PIK3CA gene andorangelabeled polynucleotides (Orange: excitation at 547 nm and emission at 572 nm,similar to rhodamine), which targets alpha satellite-sequences of the centromere ofchromosome 3.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells, circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	BioGenex eFISH PIK3CA/CEN 3 Dual Color Probemust be stored at 2-8°C, protectedfrom light and is stable through the expiry date printed on the label.

Reference	Datasheet SDS

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHR15100152	FP059-10X	10 tests	$1152		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.