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Home / Fish Probe

eFISH PDGFB Dual Color Break Apart

SKU : BHR15100141

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Mfr.No.	FP053-20X
Gene Name	PDGFB
Fluorophore	Orange/Green
Chromosome	22
Intended Use	The BioGenex eFISH PDGFB Dual Color Break Apart Probe is currently available forResearch use only.eFISH PDGFB Dual Color Break Apart Probeis designed to detecttranslocations involving the PDGFBgene at 22q13in formalin-fixed, paraffin-embeddedtissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH PDGFB Dual Color Break Apart Probe comes in hybridization buffer.The probe contains green-labeled polynucleotides (Green: excitation at 503 nm andemission at 528 nm, similar to FITC) targeting sequences in 22q13 distal to PDGFB geneand Orange labeled polynucleotides (Orange: excitation at 547 nm and emission at 572nm, similar to rhodamine), which target sequences mapping in 22q13 proximal to thePDGFB gene.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify genetic aberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acid sequences directly within a cell or tissue. High specificity is ensured through the action of annealing of fluorescence probe nucleic acid sequence to complementary target nucleic acid sequence. ISH techniques can be used to identify genetic aberrations like deletions, amplification, and translocationin tissue sections or within individual cells.
Storage	BioGenex eFISH PDGFB Dual Color Break Apart Probe must be stored at 2-8°C protected from light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100141	FP053-20X	20 Test	$1793		Add to Cart

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