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Home / Fish Probe

eFISH EWSR1 Dual Color Break Apart

SKU : BHR15100130

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Mfr.No.	FP048-10X
Gene Name	EWSR1
Fluorophore	Orange/Green
Chromosome	22
Intended Use	The BioGenex eFISH EWSR1 Dual Color Break Apart Probeis currently available for Research use only.eFISH EWSR1 Dual Color Break Apart Probeis designed to be used for the detection of translocations involving the EWSR1 gene at 22q12 in formalin-fixed, paraffinembedded tissue or cells by fluorescence in situhybridization (FISH). BioGenex eFISH EWSR1 Dual Color Break Apart Probecomes in hybridization buffer. The probe contains green-labeled polynucleotides (Green: excitation at 503 nm and emission at 528 nm, similar to FITC), which target sequences mapping in 22q12 distal to the EWSR1 gene, and orangelabeled polynucleotides (Orange: excitation at 547 nm and emission at 572 nm, similar to rhodamine), which target sequences mapping in 22q12 proximal to the EWSR1 gene.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations, presence or absence of specific DNA sequence in native context. In this technique florescent probes bind to the target sequence of DNA in chromosome. High specificity and sensitivity coupled rapid and an accurate result has proven role of FISH in both research and diagnosis of solid tumor and hematological malignancies. As technique of cancer cytogenetics, FISH, can be used to identify genetic aberrations viz., deletions, amplification and translocation in tissue sections or within individual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acid sequences directly within a cell or tissue. High specificity is ensured through the action of annealing of fluorescence probe nucleic acid sequence to complementary target nucleic acid sequence. ISH techniques can be used to identify genetic aberrations like deletions, amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH EWSR1 Dual Color Break Apart Probe must be stored at 2-8°C protected from light and is stable through the expiry date printed on the label.

Reference	Datasheet SDS

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100130	FP048-10X	10 tests	$1152		Add to Cart

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