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Home / Fish Probe

eFISH EGFR/CEN 7 Dual Color Probe

SKU : BHR15100115

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BioGenex Laboratories

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Mfr.No.	FP040-20X
Gene Name	EGFR/CEN7
Fluorophore	Orange/Green
Chromosome	7
Intended Use	The BioGenex eFISH EGFR/CEN7 dual color probe is currently available for Research use only. eFISH EGFR/CEN7 dual color probe is designed to be used for the detection of human EGFR2 geneas well aschromosome 7 alpha-satellites in formalin-fixed, paraffinembedded tissue or cells by fluorescence in situ hybridization (FISH). BioGenex eFISH EGFR/CEN7dual color probecomes in hybridization buffercontainsgreen-labeled polynucleotides (Green:excitation at 503 nm and emission at 528 nm,similar to FITC), which target the EGFR gene, andorange-labeled polynucleotides (Orange:excitation at 547 nm and emission at 572 nm,similar to rhodamine), which target alphasatellite-sequences of the centromere ofchromosome 7.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations, presence or absence of specific DNA sequence in native context. In this technique florescent probes bind to the target sequence of DNA in chromosome. High specificity and sensitivity coupled rapid and an accurate result has proven role of FISH in both research and diagnosis of solid tumor and hematological malignancies. As technique of cancer cytogenetics, FISH, can be used to identify genetic aberrations viz., deletions, amplification and translocation in tissue sections or within individual cells. FISH is also used for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific RNA targets in cells, circulating tumor cells, and tissue samples1,2,3,4,5. In an FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNA sequences. An appropriately labeled probe is hybridized to the exposed target DNA or mRNA sequences in the cells. Subsequent stringent washing steps remove any probe that is non-specifically bound to the tissue section. Subsequently slides are mounted using DAPI/antifade and can be visualized under fluorescence microscope using appropriate filter set.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acid sequences directly within a cell or tissue. High specificity is ensured through the action of annealing of fluorescence probe nucleic acid sequence to complementary target nucleic acid sequence. ISH techniques can be used to identify genetic aberrations like deletions, amplification, translocation in tissue sections or within individual cells.
Storage	The BioGenex eFISH EGFR/CEN7dual color probe must be stored at 2-8°C protected from light and is stable through the expiry date printed on the label.

Reference	Datasheet SDS

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100115	FP040-20X	20 Test	$1793		Add to Cart

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