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Home / Fish Probe

eFISH D13S319/13Q34/CEN 12 TRIPLE C

SKU : BHR15100190

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Mfr.No.	FP078-10X
Gene Name	D13S319/13Q34/CEN 12
Fluorophore	Orange/Blue
Chromosome	12
Intended Use	The BioGenex eFISHD13S319/13q34/CEN 12Triple Color Probe is currently availablefor Research use only.eFISHD13S319/13q34/CEN 12Triple Color Probe is designed to beusedfor the detection of the human D13S319 regionas well as human 13q34 specificsequences andchromosome 12 alpha-satellites in formalin-fixed,paraffin-embedded tissueor cells by fluorescencein situ hybridization (FISH).eFISHD13S319/13q34/CEN 12 TripleColor Probe is provided in hybridization buffer.Theprobe contains orange-labeled polynucleotides (Orange: excitation at 547 nm andemission at572 nm, similar to rhodamine), which target theD13S319 region, blue-labeledpolynucleotides (Blue: excitation at 418 nm and emission at467 nm, similar to DEAC),which targetchromosome 13q34 specific sequences, andgreen-labeled polynucleotides(Green:excitation at 503 nm and emission at 528 nm,similar to FITC), which target alphasatellitesequencesof the centromere of chromosome 12.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA in chromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/anti-fade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH D13S319/13q34/CEN 12Triple Color Probemust be stored at 2-8°Cprotected from light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100190	FP078-10X	10 tests	$1152		Add to Cart

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