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Home / Fish Probe

eFISH CCND1/IGH Dual Color Dual FUS

SKU : BHR15100149

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Mfr.No.	FP057-20X
Gene Name	CCND1/IGH
Fluorophore	Orange/Green
Chromosome	14
Intended Use	The BioGenex eFISH CCND1/IGH Dual Color Dual Fusion Probeis currently available for Research use only.eFISH CCND1/IGH Dual Color Dual Fusion Probeis designed to detect translocation of t(11;14)(q13;q32) in formalin-fixed, paraffin-embedded tissue or cells by fluorescence in situ hybridization (FISH). BioGenex eFISH CCND1/IGH Dual Color Dual Fusion Probecomes in Ready to use formatin hybridization buffer. The probe contains green-labeled polynucleotides (Green: excitation at 503 nm and emission at 528 nm, similar to FITC) and orangelabeled polynucleotides (Orange: excitation at 547 nm and emission at 572 nm, similar to rhodamine), which target the IGH locus in 14q32.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations, presence or absence of specific DNA sequence in native context. In this technique florescent probes bind to the target sequence of DNA in chromosome. High specificity and sensitivity coupled rapid and an accurate result has proven role of FISH in both research and diagnosis of solid tumor and hematological malignancies. As technique of cancer cytogenetics, FISH, can be used to identify genetic aberrations viz., deletions, amplification and translocation in tissue sections or within individual cells. FISH is also used for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific RNA targets in cells, circulating tumor cells, and tissue samples1,2,3,4,5. In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	BioGenex eFISH CCND1/IGH Dual Color Dual Fusion Probemust be stored at 2-8°C,protected from light and is stable through the expiry date printed on the label.

Reference	Datasheet SDS

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100149	FP057-20X	20 Test	$1793		Add to Cart

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