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Home / Fish Probe

eFISH CCND1 Dual Color Break Apart

SKU : BHR15100172

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Mfr.No.	FP069-10X
Gene Name	CCND1
Fluorophore	Orange/Green
Chromosome	11
Intended Use	The BioGenex eFISH CCND1 Dual Color Break Apart Probe is currently available forResearch use only.eFISH CCND1 Dual Color Break Apart Probeis designed to be usedfor the detection of translocations involving the chromosomal region 11q13 in formalinfixed, paraffinembedded tissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH CCND1 Dual Color Break Apart Probe comes in hybridization buffer.The probe contains green-labeled polynucleotides (Green:excitation at 503 nm andemission at 528 nm, similar to FITC), which target sequences mapping in 11q13 proximalto the CCND1 locus, and orange-labeled polynucleotides (Orange: excitation at 547 nmand emission at 572 nm, similar to rhodamine), which target sequences mapping in 11q13distal to and covering the CCND1 locus.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence in native context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH CCND1 Dual Color Break Apart Probe must be stored at 2-8°Cprotected from light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100172	FP069-10X	10 tests	$1152		Add to Cart

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