The BioGenex eFISH ALK Dual Color Break Apart Probe is currently available forResearch use only.eFISH ALK Dual Color Break Apart Probeis designed to detecttranslocations involving the ALK geneat 2p23in formalin-fixed, paraffin-embedded tissueor cells by fluorescence in situ hybridization (FISH).BioGenex eFISH ALK Dual Color Break Apart Probe comes in Ready to use formatinhybridization buffer. The probe contains green-labeled polynucleotides (Green: excitationat 503 nm and emission at 528 nm, similar to FITC) and orangelabeled polynucleotides(Orange: excitation at 547 nm and emission at 572 nm, similar to rhodamine), whichtarget sequences mapping in 2p23 proximal to the ALK breakpointregion.
Description
Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells, circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle
In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage
BioGenex eFISH ALK Dual Color Break Apart Probe must be stored at 2-8°C, protectedfrom light and is stable through the expiry date printed on the label.