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Home / Fish Probe

eFISH 1P36/1Q25 Dual Color Probe

SKU : BHR15100122

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BioGenex Laboratories

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Mfr.No.	FP044-10X
Gene Name	1p36/1q25
Fluorophore	Orange/Green
Chromosome	1
Intended Use	The BioGenex eFISH 1p36/1q25Dual Color probe is currently available for Research useonly. eFISH 1p36/1q25Dual Color probeis designed to be used for the detection ofchromosome 1p36 and 1q25 specific sequences in formalin-fixed, paraffin-embeddedtissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH 1p36/1q25Dual Color probecomes in hybridization buffer. The probecontains orange-labeled polynucleotides (Orange: excitation at 547 nm and emission at572 nm, similar to rhodamine), which target the chromosome 1p36 specific sequences,and greenlabeled polynucleotides (Green: excitation at 503 nm and emission at 528 nm,similar to FITC), which target chromosome 1q25 specificsequences.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5. In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH 1p36/1q25Dual Color probe must be stored at 2-8°C protected fromlight and is stable through the expiry date printed on the label

Reference	Datasheet SDS

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100122	FP044-10X	10 tests	$1152		Add to Cart

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