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Home / Fish Probe

eFISH 19Q13/19P13 Dual Color Probe

SKU : BHR15100124

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BioGenex Laboratories

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Mfr.No.	FP045-10X
Gene Name	19q13/19p13
Fluorophore	Orange/Green
Chromosome	19
Intended Use	The BioGenex eFISH 19q13/19p13 Dual Color Probe is currently available for Researchuse only. eFISH 19q13/19p13 Dual Color Probe is designed to be used for the detection ofchromosome 19q13 and 19p13specific sequences in formalin-fixed, paraffinembeddedtissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH 19q13/19p13 Dual Color Probe comes in hybridization buffer. Theprobe contains orange-labeled polynucleotides (Orange: excitation at 547 nm andemission at 572 nm, similar to rhodamine), which target the chromosome 19q13 specificsequences, and greenlabeled polynucleotides (Green: excitation at 503 nm and emission at528 nm, similar to FITC), which target chromosome 19p13 specificsequences.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5. In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH 19q13/19p13 Dual Color Probe must be stored at 2-8°C protectedfrom light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100124	FP045-10X	10 tests	$1152		Add to Cart

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