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Home / Fish Probe

eFISH 13/CEN 18/21 TRIPLE COLOR PRO

SKU : BHR15100222

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BioGenex Laboratories

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Mfr.No.	FP096-10X
Gene Name	13/CEN 18/21
Fluorophore	Orange/Green/Blue
Chromosome	21
Intended Use	The BioGenex eFISH SPEC 13/CEN 18/SPEC 21 TripleColor probeis currently available for Research use only.eFISHSPEC 13/CEN 18/SPEC 21. TripleColor probeis designed to be used forthe detection of human chromosome 13q12specific sequences as well as chromosome 18alphasatellites and chromosome 21q22 specificsequences in formalinfixed, paraffin-embeddedtissue or cells by fluorescence in situ hybridization(FISH). The BioGenex eFISH SPEC 13/CEN 18/SPEC 21 TripleColor probe in hybridization buffer. The probecontains green-labeled polynucleotides (Green:excitation at 503 nm and emission at 528 nm,similar to FITC), which target chromosome 13specific sequences, blue-labeled polynucleotides(Blue: excitation at 418 nm and emission at467 nm, similar to DEAC) which target alpha-satellitesequences of the centromere of chromosome18, and orange-labeled poly-nucleotides(Orange: excitation at 547 nm and emission at572 nm, similar to rhodamine), which targetchromosome 21 specific sequences.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematological malignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH SPEC 13/CEN 18/SPEC 21 TripleColor probemust be stored at 2-8°C protected from light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100222	FP096-10X	10 tests	$1152		Add to Cart

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