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Alu II Probe

SKU : BHR15100240

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BioGenex Laboratories

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Mfr.No.	PR026-100
Intended Use	BioGenex ALU II PROBE is currently available for in vitro diagnostic use. This probe is designed for the specific localization of Alu II DNA in formalin-fixed, paraffin-embedded human tissues and/or frozen tissues by in situ hybridization.
Description	The Alu II probe detects Alu repeat sequences of human DNA present in formalinfixed, paraffin-embedded human tissues by in situ hybridization. Alu, an important group of widely distributed sequence repeats in the human genome, provides a positive control for in situ hybridization detection system. BioGenex automated in situ hybridization technique will allow the detection of Alu in various human tissues.
Principle	In situ hybridization (ISH) allows the detection and localization of definitive nucleic acid sequences directly within a cell or tissue. High specificity is ensured through the action of annealing of probe nucleic acid sequence to complementary target nucleic acid sequence. ISH techniques can be used to identify infectious agents in tissue sections, to localize gene expression within individual cells, or to detect specific DNA sequences in the genome of cells. In ISH, fixed tissue sections are deproteinized to expose target DNA or mRNA sequences. A hapten (fluorescein or digoxigenin-labeled probe) is hybridized to the exposed target DNA or mRNA sequences in the cells. Subsequent washing steps remove any probe that is not bound or that is non-specifically bound to the tissue section. An immunohistochemical (IHC) procedure is then used to detect the probetarget hybrid. (Down stream detection of hybridized hapten labeled probe is done by using specific anti-hapten antibody). This procedure includes incubating the slide with a mouse anti- fluorescein or digoxigenin antibody, followed by detection of this antibody with a second antibody enzyme conjugate. After addition of an appropriate substrate for the enzyme (such as DAB,-diaminobenzidine-solution), a colored reaction product is precipitated at the location of the probe-target hybrid. Microscopic examination of the slide provides visual interpretation of the staining results.
Storage	Store the probe at 2-8o C. Warm to room temperature immediately prior to use (HPV probes may need water baths higher than 37o C to dissolve the precipitate in the probe). This probe is suitable for use till expiry date when stored at 2-8°C. Do not use the product after expiration date printed on vial. If reagents are stored under any conditions other than those specified in the package insert, they must be verified by the user. Positive and negative controls should be run simultaneously for every experiment. If unexpected staining is observed which cannot be explained by variations in laboratory procedures and a problem with the antibody is suspected, contact BioGenex Technical Support at 1-800-421-4149 or your local distributor.

Reference	Datasheet SDS

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100240	PR026-100	25 Tests - Manual	$365		Add to Cart

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