Restriction enzyme digestion of double-stranded DNA
Components
XhoI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Impaired
Concentration
20000 U/ml
Heat Inactivation
65°C for 20 min
Quantity
10000 U (500ul)
Recognition Site
Sequence C^TCGAG
Source
An E. coli strain that carries the cloned and modified XhoI gene from Xanthomonas holcicola (ATCC 13461). The XhoI enzyme recognizes and cuts the sequence C^TCGAG.