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Home / Enzymes, Inhibitors & Substrates

T7 RNA Polymerase

SKU : BHZ10900044

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E041
Description	T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences.
Description	Synthesis of RNA transcripts for hybridization probes ; Synthesis of RNA for in vitro translation ; Synthesis of biologically active mRNA ; Generate large amounts of labelled or non-labelled RNA
Components	Enzyme supplied with 10X Reaction Buffer
Notes	One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer.
Concentration	50 U/ul
Quantity	100 ul (5000 U)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM β-ME, 100 mM NaCl, 0.1% Triton®X-100 and 50% (v/v) Glycerol.
Format	Polymerase
Concentration	50 U/ul

Protocol	T7 RNA Polymerase Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900044	E041	100µl	$84.45		Add to Cart

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