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Home / Enzymes, Inhibitors & Substrates

SpeI

SKU : BHZ10900089

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E075
Description	Restriction enzyme digestion of double-stranded DNA
Components	SpeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes	Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
Concentration	10000 U/ml
Heat Inactivation	80°C for 20 min
Quantity	2500 U (250 µl)
Recognition Site	Sequence A^CTAGT
Source	An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). The SpeI enzyme recognizes and cuts the sequence A^CTAGT.

Storage	-20°C
Shipping Condition	Ice Packs
Storage Condition	-20C
Buffer	Storage Buffer: 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 200 µg/ml BSA, 250 mM NaCl, 0.15% Triton®X-100, pH 7.4 @ 25°C. Reaction Buffer: Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%
Format	Endonuclease
Concentration	10000 U/ml

QC	Test Study- Restriction Enzyme Stability
Protocol	E075 Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900089	E075	250µl	$386		Add to Cart

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