Restriction enzyme digestion of double-stranded DNA
Components
SalI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping
Concentration
20000 U/ml
Heat Inactivation
65°C for 20 min
Quantity
10000 U (500 µl)
Recognition Site
Sequence G^TCGAC
Source
An E. coli strain that carries the modified SalI gene from Streptomyces albus G (ATCC 49789). The SalI enzyme recognizes and cuts the sequence G^TCGAC.
Storage
-20°C
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) GlycerolReaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 100%, Universal Buffer: 100%