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Home / Enzymes, Inhibitors & Substrates

RNase R

SKU : BHZ10900085

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E049
Description	RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven nucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.
Description	Enriching circRNAs in biological samples ; Identification of intronic lariat sequences ; Identification of exonic circRNAs ; Studying alternative splicing ; Production of artificial circular RNAs
Notes	1) If degradation is inefficient, use a slightly higher incubation temperature (40-45°C) and supplement additional enzyme partway (e.g. 0.5 µl after 1 hour) through the procedure. The higher temperature is particularly useful for degrading highly structured linear RNAs, such as rRNAs. Do not exceed 45°C or incubate over 3 hours, as this may lead to non-enzymatic RNA degradation. 2) Magnesium at concentrations of 0.1-1.0 mM is required for optimal activity. If EDTA is present, compensate by adding MgCl2 to 1.0 mM final. 3) RNase R exhibits low activity on tRNA, rRNA and other highly structured RNAs, for which the 3’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and result in greatly reduced activity. For best results, removal of rRNA from total RNA extracts is highly recommended. One unit is defined as the amount of RNase R that converts 1 µg of poly(A) into acid-soluble nucleotides in 10 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	20 U/μl
Form	Enzyme supplied with 10X Reaction Buffer.
Quantity	500 U (25 μl)
Source	Recombinant E. coli

Storage	Store all components at -20 °C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.
Shipping Condition	Ice Packs
Storage Condition	-20C
Buffer	Storage Buffer: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol. Reaction Buffer: 200 mM Tris-HCl, 1 M KCl, 1 mM MgCl2, pH 7.5
Format	Ribonuclease
Concentration	20 U/μl

Protocol	RNase R Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900085	E049	50µl	$295		Add to Cart

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