Restriction enzyme digestion of double-stranded DNA
Components
PstI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
Concentration
20000 U/ml
Heat Inactivation
No
Quantity
20000 U (1 ml)
Recognition Site
Sequence CTGCA^G
Source
An E. coli strain that carries the cloned and modified PstI gene from Providencia stuartii164 (ATCC 49762). The PstI enzyme recognizes and cuts the sequence CTGCA^G.