ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects.
Description
transformation efficiency: >1x10^6 cfu/µg with abm’s expression vectors (>10kb in size). ; ideal for routine plasmid amplification. ; reduces recombination and improves yield and quality of plasmid DNA prepared from minipreps; can be sequenced directly. ; not Suitable for preparing unmethylated DNA.