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Home / Enzymes, Inhibitors & Substrates

Poly(A) Polymerase, Yeast

SKU : BHZ10900002

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E017
Description	Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
Description	Labelling of RNA with ATP or cordycepin ; Poly(A) tailing of RNA for cloning or affinity purification ; Increasing translation of RNA transferred into eukaryotic cells
Components	Enzyme supplied with 5X Reaction Buffer.
Notes	One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use .
Concentration	1 U/μl
Quantity	100 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Format	Polymerase
Concentration	1 U/μl

Supporting Protocol	Poly(A) Polymerase, Yeast Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900002	E017	100µl	$73.85		Add to Cart

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