Klenow Fragment (3’→5’ Exo-)

Mfr.No.	E038
Description	Klenow Fragment (3’→5’ Exo-) is the large fragment of E. coli DNA Polymerase I which retains polymerase activity but lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity.
Description	Dideoxy DNA sequencing of single- or double-stranded DNA templates ; cDNA second-strand synthesis ; Generate single-stranded DNA probes using random primers ; Site-directed DNA mutagenesis using synthetic oligonucleotides
Notes	One unit is defined as the amount of Klenow Fragment (3’→5’ Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	5 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	500 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Ice Packs
Storage Condition	-20C
Buffer	Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
Format	Polymerase
Concentration	5 U/μl

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900046	E038	100µl	$58.85		Add to Cart