Hieff™ Gold T4 DNA Ligase (5 U/μL)

Mfr.No.

10300ES80, 10300ES97

Description

Hieff™ Gold T4 DNA Ligase can catalyze the formation of phosphodiester bonds at the 5' phosphate end and 3' hydroxyl end of adjacent nucleic acids at blunt or sticky ends of dsDNA when ATP is used as a coenzyme, and can also catalyze RNA to link with ssDNA or ssRNA in double strands, but cannot catalyze linkages between fully single-stranded nucleotides. This product is suitable for labeling the 3'-end of RNA, circularizing RNA and DNA oligonucleotides, cloning cDNA, etc.

Enzyme	Ligase
Product Type	T4 DNA Ligase

Components

Components No.	Name	10300ES80(1,000 U)
10300-A	Hieff™ Gold T4 DNA Ligase (5 U/µL)	200 µL
10300-B	10×Ligase Buffer	400 µL

Notes

It is suitable for nucleic acid operations such as labeling RNA 3 '-end, looping RNA and DNA oligonucleotides, and cloning cDNA.

Concentration

5 U/µL

Applications Range	Cloning (Blunt or sticky end joining) A linker or adaptor can be added to the flat-end DNA

Storage	The product should be stored at -25°C~-15°C for two years. Please avoid repeated freeze-thaw.
Format	Ligase
Concentration	5 U/µL

Protocals And Manuals	10300_MSDS_HB220423_EN_1652931562604.PDF 10300_MSDS_HB220423_EN_1652931562605.PDF Manual

Ligation of Lambda DNA digest using Hieff Gold T4 DNA ligase

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHZ20800015-1	10300ES80	1000U	$85		Add to Cart
BHZ20800015-2	10300ES97	50x1000U	$2750		Add to Cart

Hieff™ Gold T4 DNA Ligase (5 U/μL)

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