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Home / Enzymes, Inhibitors & Substrates

E. coli DNA Ligase

SKU : BHZ10900099

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E007
Description	E.coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not effective for the formation of DNA-RNA or RNA-RNA hybrids.
Description	Ligation of dsDNA with cohesive termini ; cDNA cloning of products from second strand cDNA synthesis experiments ; Alternative to T4 DNA Ligase when blunt-end ligation is not required ; For ligation of blunt-end fragments use T4 DNA Ligase (abm Cat. No. G467) ; Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Notes	One unit is defined as the amount of E. coli DNA Ligase that is required to give 50% ligation of HindIII-digested λ DNA in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E.coli DNA Ligase Reaction Buffer (concentration of 5′ DNA termini is 0.12 μM [300 μg/ml]). This product is distributed for laboratory research only. Caution: Not for diagnostic use .
Concentration	10 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	1000 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM DTT, 200 mM NaCl, and 50% (v/v) Glycerol.
Format	Ligase
Concentration	10 U/μl

Protocol	E. coli DNA Ligase Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900099	E007	100µl	$89.75		Add to Cart

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