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Home / Enzymes, Inhibitors & Substrates

DpnI

SKU : BHZ10900086

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E026
Description	The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated.
Description	Molecular cloning ; Site directed mutagenesis ; Restriction site mapping ; Genotyping ; Southe Blot ; SNP ; Restriction fragment length polymorphism (RFLP)
Notes	One unit is defined as the amount of Dpn1 required to digest 1 µg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	20 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	2000 U (100 μl)

Storage	-20°C
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 µg/ml BSA, and 50% (v/v) Glycerol.
Format	Endonuclease
Concentration	20 U/μl

QC	Test Study- Restriction Enzyme Stability
Protocol	Protocol 1

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900086	E026	100µl	$97.45		Add to Cart

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