The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One conce with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease.
Notes
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Alternative Names
Cas9H840A, Cas9(H840A), CRISPR-associated endonuclease Cas9 (H840A) from Streptococcus pyogenes
Concentration
1000 nM, 160 µg/ml
Form
Enzyme supplied with 10X Reaction Buffer
Quantity
8.0µg (50pmol); Volume: 50µL
Source
E. coli
Storage
Store all components at -20°C.
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.