The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease.
Notes
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Alternative Names
Cas9n, spCas9n, Cas9D10A, Cas9(D10A), CRISPR-associated endonuclease Cas9(D10A) from Streptococcus pyogenes
Concentration
10 µM, 1.60 mg/ml
Form
Enzyme supplied with 10X Reaction Buffer
Quantity
40µg (250pmol); Volume: 25µL
Source
E. coli
Storage
Store all components at -20°C.
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.