Bsu DNA Polymerase, Large Fragment is from Bacillus subtilis and is modified so that it retains its 5’→ 3’ DNA polymerase activity with its 5’→ 3’ exonuclease domain removed. This DNA polymerase naturally lacks the 3’→ 5’ exonuclease activity.
Description
Primer labelling ; Second strand cDNA synthesis ; DNA strand displacement synthesis
Notes
One unit is defined as the amount of Bsu DNA Polymerase, Large Fragment that that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 °C.
This product is distributed for laboratory research only Caution: Not for diagnostic use.
Concentration
5 U/μl
Form
Enzyme supplied with 10X Reaction Buffer.
Quantity
1000 U (200 μl)
Storage
Store all components at -20°C.
Shipping Condition
Dry Ice
Storage Condition
-20C
Buffer
Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 50 mM NaCl, and 50% (v/v) Glycerol.