Restriction enzyme digestion of double-stranded DNA
Components
BglII enzyme supplied with 10X Restriction Enzyme Reaction Buffer 3
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
Concentration
10000 U/ml
Heat Inactivation
No
Quantity
5000 U (500ul)
Recognition Site
Sequence A^GATCT
Source
AnE. coli strain that carries the cloned and modified BglII gene from Bacillus globigii (ATCC 4976). The BglII enzyme recognizes and cuts the sequence A^GATCT.
Storage
-20°C
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 50 mM KCl,200 µg/ml BSA, pH 7.4 @ 25°C.Reaction Buffer: Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%