Restriction enzyme digestion of double-stranded DNA
Components
AvrII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
Concentration
5000 U/ml
Heat Inactivation
No
Quantity
500 U (100 µl)
Recognition Site
Sequence C^CTAGG
Source
An E. coli strain that carries the cloned and modified AvrII gene from Anabaena variabilis UW (E. Rosenvold). The AvrII enzyme recognizes and cuts the sequence C^CTAGG.
Storage
-20°C
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, pH 7.4 @ 25 °C.Reaction Buffer: Buffer 1: 100%, Buffer 2: 50%, Buffer 3: 50%, Universal Buffer: 100%