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Home / Enzymes, Inhibitors & Substrates

asCpf1 Nuclease NLS Protein

SKU : BHZ10900137

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Applied Biological Materials (abm) Inc.

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Mfr.No.	K088
Description	Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases. Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. Cpf1 can be used with a shorter guide RNA (called crRNA) than Cas9. Cpf1 creates a staggered cut in dsDNA instead of a blunt cut. Cpf1 cuts distal to the PAM sequence, which may allow for multiple rounds of cleavage. asCpf1 is from the bacteriaAcidaminococcus. This protein contains a SV40 T antigen nuclear localization signal (NLS) on the N-terminus of the protein. If the cut caused by asCpf1 is repaired by non-homologous end joining (NHEJ), an indel may be formed that disrupts the open reading frame of the targeted gene, leading to gene knockout. Alteatively, by supplying a repair template, a sequence can be knocked in at the cleavage site via homology directed repair (HDR).
Notes	This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Alternative Names	Cas12a ; asCas12a ; Cpf1 fromAcidaminococcus ; Cas12a fromAcidaminococcus
Concentration	10 μM, 1.5 mg/ml
Form	Enzyme supplied with 10X Reaction Buffer.
Source	E. coli

Storage	Store all components at -20°C.
Shipping Condition	Ice Packs
Storage Condition	-20C
Storage Buffer	10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Concentration	10 μM, 1.5 mg/ml

Supporting Protocol	asCpf1 Protein DatasheetNotes: 18092018

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900137	K088	25µl	$73.89		Add to Cart

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