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Home / Enzymes, Inhibitors & Substrates

ApaI

SKU : BHZ10900011

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E063
Description	Restriction enzyme digestion of double-stranded DNA
Components	ApaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes	Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Blocked by overlapping CpG Methylation: Blocked by overlapping
Concentration	50000 U/ml
Heat Inactivation	65°C for 20 min
Quantity	25000 U (500 µl)
Recognition Site	Sequence GGGCC^C
Source	An E. coli strain that carries the ApaI gene from Acetobacter pasteurianus sub. pasteurianus (ATCC 9432). The ApaI enzyme recognizes and cuts the sequence GGGCC^C.

Storage	-20°C
Shipping Condition	Ice Packs
Storage Condition	-20C
Buffer	Storage Buffer: 10mM Tris pH (7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol. Reaction Buffer: Buffer 1: 25%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%
Format	Endonuclease
Concentration	50000 U/ml

QC	Test Study- Restriction Enzyme Stability
Supporting Protocol	E063 Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900011	E063	500µl	$144.44		Add to Cart

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