Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked
Concentration
20000 U/ml
Heat Inactivation
65°C for 20 min
Quantity
1500 U (75 µl)
Recognition Site
Sequence A^CCGGT
Source
An E. coli strain that carries the cloned and modified AgeI gene from Ruegeria gelatinovora (ATCC 25655). The AgeI enzyme recognizes and cuts the sequence A^CCGGT.
Storage
-20°C
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 200 µg/ml BSA, 250 mM NaCl, 0.15% Triton® X-100, pH 7.4 @25°C