| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological (milk), food and agriculture, etc |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. The assay uses OD570nm, or FL530/585nm for signal readout. Compatible sample input includes Biological (milk), food and agriculture, etc. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological (milk), food and agriculture, etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 6 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays.
Additional feature notes highlight Convenient and high-throughput. Robust and homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of lactose within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct, and high-throughput assays for lactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 6 µM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify lactose in biological (milk) by OD570 nm, or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched biological (milk) handling.
- Monitor time-course or pre/post changes in biological (milk) across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
I would like to know if I can use the optical density at 540nm to read my samples.
The signal at 540nm is only about 2/3 of the signal at 570 nm, but you still can use it. However, you will lose sensitivity.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Lactose-induced chronic diarrhea results from abnormal luminal microbial fermentation and disorder of ion transport in the colon
Xue, H et al. (2020). Lactose-induced chronic diarrhea results from abnormal luminal microbial fermentation and disorder of ion transport in the colon. Frontiers in Physiology, 11, 877. Assay: Lactose in rat feces.
Milk biosynthesis requires the Golgi cation exchanger TMEM165
Snyder, N. A., Palmer, M. V., Reinhardt, T. A., & Cunningham, K. W. (2019). Milk biosynthesis requires the Golgi cation exchanger TMEM165. Journal of Biological Chemistry, 294(9), 3181-3191. Assay: Lactose in mouse skimmed milk.
Liposome surface functionalization based on different anchoring lipids via Staudinger ligation
Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44. Assay: Lactose in liposome.
Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats
Kwon DH et al (2012).Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. J Nutr Biochem 23(7):791-9. Assay: Lactose in rat milk.
