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Home / Assay Kits / ELISA Kits

SAH ELISA 3

SKU : BHE16600019

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Arthus Biosystems

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Mfr.No.	IK00303
Description	Principle of the assay : This direct competitive ELISA (Enzyme Linked lmmunosorbent assay) is designed to measure the level of S-adenosyl-L-methionine (SAH) in the sample. SAH conjugated with macromolecule is immobilized on the micro-titer plate. Standards and samples are pipetted into the wells, and then the HRP-conjugated antibody against SAH is added. The free SAH molecule in samples or standards competes with the immobilized SAH on the micro-titer plate surface for binding sites of the antibody. After discarding the mixed solution and washing each well, TMB substrate solution is added. The substrate solution turns blue under the effect of HRP (horseradish peroxidase), and changes into yellow once stop solution (acid) is added. The color develops in inverse proportion to the amount of SAH in the sample (or standards). The optical density of the remaining solution (00450) is measured at 450nm using micro-plate spectrophotometer. The level of SAH in samples can be calculated through standard curve generated with standards. Sample collection and storage : 1. Sample collection must be carried out at 4 °C. Remove precipitation by centrifugation when necessary, and test sample as soon as possible or store at -20°C or below. Avoid repeated freeze-thaw cycles. 2. Avoid NaN3 as it will inactivate HRP.
Notes	1. Using reagents and samples without warm-up or ambient temperature less than 20°C may lead to reduced 0D450 reading 2. Extra drying of wells after washing may have negative effects on the results, such as poor standard curve and poor repeatability. To avoid it, perform the next step immediately after washing. 3. Mix solution well and wash completely, as these procedures will have influence on the assay. 4. Seal the plate with micro-plate sealer and avoid light when incubating the plate. 5. Duplicate wells for standards and samples are recommended, and quadratic polynomial is suggested for standard curve fitting (R2>0.99). The detected concentration of quality control vial should be in the detection range. 6. The concentrated wash solution may form crystals. Warm it up to allow salts to dissolve completely before diluting 7. The micro-plate sealers should be disposed after each use in order to avoid cross contaminations. 8. The HRP substrate (TMB) should be kept in dark 9. The Stop Solution is diluted sulfuric acid. Avoid direct contact with skin and other things.

Target	Methionine is an amino acid with sulfur methyl group, which is converted into S-adenosylmethionine (SAM) with ATP by methionine adenosyltransferase (EC 2.5.1.6). SAM is the sole methyl group donor for over 50 kinds of biologically active substances such a

Storage	Storage condition: Except for the HRP substrate and stop solution that are stored at 2-8°C, all other ingredients and strips can be frozen stored. Expiration: About 6 months from the date of shipment when storing it in refrigerator. If not planning to use

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SKU	Supplier No.	Size	Your price	Quantity
BHE16600019	IK00303	48 tests	$499		Add to Cart

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